Composite

Part:BBa_K4688007:Design

Designed by: Xiaoxin Xuan   Group: iGEM23_FZPt-CHINA   (2023-10-06)


FUCO3-GFP-expression


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2347
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal BamHI site found at 3498
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2347
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2347
    Illegal NgoMIV site found at 2081
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 999
    Illegal BsaI site found at 1202


Design Notes

Codon optimization and gene expression efficiency.


Source

FUCO3 comes from chromosome 19 of the Phaeodactylum tricornutum genome.GFP comes from aequoreavictoria.

References